The mk2 peptide & protein purification platform
mk2 has developed a disruptive platform technology that enables the purification of recombinantly produced peptides & proteins at low-costs and high-purity. The process is completely chromatography- and resin-free and is performed in an aqueous environment. This makes the process highly scalable and cost-effective allowing for easy and efficient large-scale production.
How does it work in principle?
The purification process involves an engineered proprietary fusion protein, containing the product either at the N-or at the C-terminus to best meet the technical specifications of the product of interest. The fusion protein consists of a cleavage domain and a non-affinity-based purification tag. At first the fusion protein is isolated from the host cell impurities by employing the purification tag. Post this, the product of interest is cleaved off the tag, and the tag is removed using the same principles, allowing for the protein to remain in solution.
| Conventional downstream process
Crude Product
Chromatography
Purified product
Characteristics
01
Low-throughput processing
02
Lack of scalability
03
Low yields/recoveries
04
Expensive & toxic materials
| Conventional downstream process
Crude Product
Chromatography
Purified product
Characteristics
01
Low-throughput processing
02
Lack of scalability
03
Low yields/recoveries
04
Expensive & toxic materials
| mk2 downstream process
Crude Product
Stirred tanks, filtration & water-based buffers
